Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2

J Dent Res. 2004 Aug;83(8):590-5. doi: 10.1177/154405910408300802.


Regenerative medicine is based on stem cells, signals, and scaffolds. Dental pulp tissue has the potential to regenerate dentin in response to noxious stimuli, such as caries. The progenitor/stem cells are responsible for this regeneration. Thus, stem cell therapy has considerable promise in dentin regeneration. Culture of porcine pulp cells, as a three-dimensional pellet, promoted odontoblast differentiation compared with monolayers. The expression of dentin sialophosphoprotein (Dspp) and enamelysin/matrix metalloproteinase 20 (MMP20) mRNA confirmed the differentiation of pulp cells into odontoblasts and was stimulated by the morphogenetic signal, bone morphogenetic protein 2 (BMP2). Based on the in vitro experiments, an in vivo evaluation of pulp progenitor/stem cells in the dog was performed. The autogenous transplantation of the BMP2-treated pellet culture onto the amputated pulp stimulated reparative dentin formation. In conclusion, BMP2 can direct pulp progenitor/stem cell differentiation into odontoblasts and result in dentin formation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins / administration & dosage*
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Culture Techniques / methods
  • Dental Pulp / cytology
  • Dental Pulp / drug effects*
  • Dental Pulp Capping / methods
  • Dental Pulp Cavity / surgery
  • Dentin / drug effects
  • Dentin / physiology*
  • Dentin / surgery
  • Dentinogenesis / drug effects
  • Dentinogenesis / physiology
  • Dogs
  • Humans
  • Odontoblasts / drug effects
  • Recombinant Proteins
  • Regeneration / drug effects*
  • Regeneration / physiology
  • Stem Cell Transplantation / methods*
  • Stem Cells / cytology
  • Stem Cells / drug effects*
  • Swine
  • Transforming Growth Factor beta*


  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • recombinant human bone morphogenetic protein-2