Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Aug;53(Pt 8):783-786.
doi: 10.1099/jmm.0.05425-0.

A Fast, Practical and Reproducible Procedure for the Standardization of the Cell Density of an Aspergillus Suspension

Affiliations

A Fast, Practical and Reproducible Procedure for the Standardization of the Cell Density of an Aspergillus Suspension

Ricardo Araujo et al. J Med Microbiol. .

Abstract

The progressive increase of invasive disease and reports of resistance among Aspergillus species emphasizes the need for reproducible antifungal susceptibility testing. Inoculum standardization is a crucial step in such procedures. The objective of this study was to develop a fast and precise method of evaluating the cell density of an Aspergillus spore suspension, as an alternative to spectrometric readings or cell-counting with a haemocytometer. Densimat (bioMérieux) is a portable photometer that shows a good correlation with spectrometric readings and can advantageously replace the cumbersome, time-consuming method of cell-counting. Thus, Densimat brings significant improvement to the reproducibility and feasibility of standardization of a fungal inoculum.

Similar articles

See all similar articles

Cited by 10 articles

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources

Feedback