Standardization of the Method for Estimation of Ethambutol in Pharmaceutical Preparations and Biological Fluid

Indian J Exp Biol. 2004 Jan;42(1):68-73.

Abstract

A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an organic solvent, followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 microg/ml. The relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine quantitatively and stable for a period of at least one week in urine stored at -20 degrees C.

MeSH terms

  • Antitubercular Agents / pharmacokinetics*
  • Antitubercular Agents / urine*
  • Chemistry, Clinical / methods*
  • Chromatography / methods*
  • Dose-Response Relationship, Drug
  • Ethambutol / pharmacokinetics*
  • Ethambutol / urine*
  • HIV Seropositivity / complications
  • HIV Seropositivity / drug therapy
  • Humans
  • Isoniazid / chemistry
  • Pharmaceutical Preparations*
  • Pyrazinamide / chemistry
  • Reference Standards
  • Reproducibility of Results
  • Resins, Synthetic / chemistry
  • Sensitivity and Specificity
  • Time Factors

Substances

  • Antitubercular Agents
  • Pharmaceutical Preparations
  • Resins, Synthetic
  • Pyrazinamide
  • Ethambutol
  • amberlite
  • Isoniazid