Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells

J Gen Microbiol. 1992 Aug;138 Pt 8:1689-96. doi: 10.1099/00221287-138-8-1689.


Twenty-five strains of lactobacilli were tested for their ability to adhere to human enterocyte-like Caco-2 cells in culture. Seven Lactobacillus strains adhered well to the Caco-2 cells, of which three possessed calcium-independent adhesion properties. A high level of calcium-independent adhesion was observed with the human stool isolate Lactobacillus acidophilus strain LB. Scanning electron microscopy revealed that this strain adhered to the apical brush border of the cells. Adhesion increased in parallel with the morphological and functional differentiation of the Caco-2 cells. Two Lactobacillus components were involved in this adhesion. One was protease-resistant and bacterial-surface-associated; the other was heat-stable, extracellular and protease-sensitive.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Adhesion*
  • Binding Sites
  • Cell Differentiation
  • Cell Line
  • Humans
  • Intestines / cytology
  • Intestines / microbiology*
  • Kinetics
  • Lactobacillus acidophilus / physiology*