The expression of B7-H1 on keratinocytes in chronic inflammatory mucocutaneous disease and its regulatory role

Immunol Lett. 2004 Jul 15;94(3):215-22. doi: 10.1016/j.imlet.2004.05.007.


PD-1 and its ligands, B7-H1/PD-L1 and B7-DC/PD-L2, have been identified recently as CD28-B7 family molecules that are implicated in immune regulation. Lichen planus (LP) is a T cell-mediated chronic inflammatory mucocutaneous disease. We investigated the expression and function of PD-1 and its two ligands in LP. Immunohistochemical examination revealed the abundant expression of PD-1 and B7-H1 in infiltrating T cells and macrophages, and lower-level expression of B7-DC on macrophages in the subepithelium. Interestingly, substantial expression of B7-H1 on keratinocytes (KCs) was found close to the numerous T cell infiltrates in the subepithelium. Unstimulated cultured KCs expressed both B7-H1 and B7-DC, and their expression was upregulated by proinflammatory cytokines, particularly IFN-gamma. The T-cell proliferative responses and IFN-gamma production that were induced by IFN-gamma-treated KCs were augmented preferentially by anti-B7-H1 mAb, but not by anti-B7-DC mAb. These results indicate the regulatory role of B7-H1 on KCs in the interactions with T cells. Our results suggest that the induction of B7-H1 on KCs may play an important role in tolerance induction in the inflamed oral mucosa and skin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, CD
  • Antigens, Surface / immunology
  • Antigens, Surface / metabolism
  • Apoptosis Regulatory Proteins
  • B7-1 Antigen / immunology
  • B7-1 Antigen / metabolism*
  • B7-H1 Antigen
  • Female
  • Humans
  • Immunohistochemistry
  • Keratinocytes / immunology
  • Keratinocytes / metabolism*
  • Lichen Planus, Oral / immunology
  • Lichen Planus, Oral / metabolism*
  • Male
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / metabolism*
  • Middle Aged
  • Peptides / immunology
  • Peptides / metabolism*
  • Programmed Cell Death 1 Ligand 2 Protein
  • Programmed Cell Death 1 Receptor
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism


  • Antigens, CD
  • Antigens, Surface
  • Apoptosis Regulatory Proteins
  • B7-1 Antigen
  • B7-H1 Antigen
  • CD274 protein, human
  • Membrane Glycoproteins
  • PDCD1 protein, human
  • PDCD1LG2 protein, human
  • Peptides
  • Programmed Cell Death 1 Ligand 2 Protein
  • Programmed Cell Death 1 Receptor