Abstract
The glycine transporter subtype 2 (GlyT2) is localized at glycinergic axon terminals where it mediates the re-uptake of glycine from the extracellular space. In this study, we used the yeast two-hybrid system to search for proteins that interact with the cytoplasmic carboxy terminal tail region of GlyT2. Screening of a rat brain cDNA library identified the PDZ domain protein syntenin-1 as an intracellular binding partner of GlyT2. In pull-down experiments, the interaction between GlyT2 and syntenin-1 was found to involve the C-terminal amino acid residues of GlyT2 and the PDZ2 domain of syntenin-1. Syntenin-1 is widely expressed in brain and co-localizes with GlyT2 in brainstem sections. Furthermore, syntenin-1 binds syntaxin 1A, which is known to regulate the plasma membrane insertion of GlyT2. Thus, syntenin-1 may be an in vivo binding partner of GlyT2 that regulates its trafficking and/or presynaptic localization in glycinergic neurons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence / genetics
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Amino Acid Transport Systems, Neutral / genetics
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Amino Acid Transport Systems, Neutral / metabolism*
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Animals
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Antigens, Surface / metabolism
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Binding Sites / genetics
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Brain / cytology
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Brain / metabolism*
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Line
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Cell Membrane / genetics
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Cell Membrane / metabolism
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Glycine / metabolism*
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Glycine Plasma Membrane Transport Proteins
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Humans
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Immunohistochemistry
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Male
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Nerve Tissue Proteins / metabolism
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Neurons / cytology
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Neurons / metabolism*
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Presynaptic Terminals / metabolism
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Protein Binding / genetics
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Protein Structure, Tertiary / genetics
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Protein Transport / genetics
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Rats
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Rats, Wistar
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Syntaxin 1
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Syntenins
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Two-Hybrid System Techniques
Substances
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Amino Acid Transport Systems, Neutral
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Antigens, Surface
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Carrier Proteins
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Glycine Plasma Membrane Transport Proteins
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Nerve Tissue Proteins
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SLC6A5 protein, human
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STX1A protein, human
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Sdcbp protein, rat
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Slc6a5 protein, rat
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Stx1a protein, rat
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Syntaxin 1
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Syntenins
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Glycine