Abstract
ACE2 purified from failing human heart was found to form a complex with integrin beta1 by immunoprecipitation, Western blotting, activity assay, and ESI tandem mass spectroscopy. The ACE2/integrin complex showed a Km of 6.8 microM and a Vmax of 2.13 pmol/min/microl purified enzyme. Activity was optimal at pH 7.5 with Ang II substrate.
MeSH terms
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Amino Acid Sequence
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Angiotensin-Converting Enzyme 2
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Carboxypeptidases / metabolism*
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Cardiomyopathies / metabolism*
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Humans
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Integrin beta1 / metabolism*
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Mass Spectrometry
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Molecular Sequence Data
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Peptidyl-Dipeptidase A
Substances
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Integrin beta1
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Carboxypeptidases
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Peptidyl-Dipeptidase A
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ACE2 protein, human
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Angiotensin-Converting Enzyme 2