Interaction of ACE2 and integrin beta1 in failing human heart

Qishan Lin; Rebecca S Keller; Barbara Weaver; Lawrence S Zisman

doi:10.1016/j.bbadis.2004.05.005

Interaction of ACE2 and integrin beta1 in failing human heart

Biochim Biophys Acta. 2004 Aug 4;1689(3):175-8. doi: 10.1016/j.bbadis.2004.05.005.

Authors

Qishan Lin¹, Rebecca S Keller, Barbara Weaver, Lawrence S Zisman

Affiliation

¹ The Center for Functional Genomics, Proteomics Core Facility, University at Albany, Rensselaer, NY, USA.

PMID: 15276642
DOI: 10.1016/j.bbadis.2004.05.005

Abstract

ACE2 purified from failing human heart was found to form a complex with integrin beta1 by immunoprecipitation, Western blotting, activity assay, and ESI tandem mass spectroscopy. The ACE2/integrin complex showed a Km of 6.8 microM and a Vmax of 2.13 pmol/min/microl purified enzyme. Activity was optimal at pH 7.5 with Ang II substrate.

MeSH terms

Amino Acid Sequence
Angiotensin-Converting Enzyme 2
Carboxypeptidases / metabolism*
Cardiomyopathies / metabolism*
Humans
Integrin beta1 / metabolism*
Mass Spectrometry
Molecular Sequence Data
Peptidyl-Dipeptidase A

Substances

Integrin beta1
Carboxypeptidases
Peptidyl-Dipeptidase A
ACE2 protein, human
Angiotensin-Converting Enzyme 2