Interaction of ACE2 and integrin beta1 in failing human heart

Biochim Biophys Acta. 2004 Aug 4;1689(3):175-8. doi: 10.1016/j.bbadis.2004.05.005.

Abstract

ACE2 purified from failing human heart was found to form a complex with integrin beta1 by immunoprecipitation, Western blotting, activity assay, and ESI tandem mass spectroscopy. The ACE2/integrin complex showed a Km of 6.8 microM and a Vmax of 2.13 pmol/min/microl purified enzyme. Activity was optimal at pH 7.5 with Ang II substrate.

MeSH terms

  • Amino Acid Sequence
  • Angiotensin-Converting Enzyme 2
  • Carboxypeptidases / metabolism*
  • Cardiomyopathies / metabolism*
  • Humans
  • Integrin beta1 / metabolism*
  • Mass Spectrometry
  • Molecular Sequence Data
  • Peptidyl-Dipeptidase A

Substances

  • Integrin beta1
  • Carboxypeptidases
  • Peptidyl-Dipeptidase A
  • ACE2 protein, human
  • Angiotensin-Converting Enzyme 2