Preparation and biological activity of molecular probes to identify and analyze jasmonic acid-binding proteins

Biosci Biotechnol Biochem. 2004 Jul;68(7):1461-6. doi: 10.1271/bbb.68.1461.

Abstract

Several types of jasomonic acid (JA) derivatives, including JA--amino acid conjugates, a JA--biotin conjugate, a JA--dexamethasone heterodimer, and a JA-fluoresceine conjugate, were prepared as candidates for molecular probes to identify JA--binding proteins. These JA derivatives, excepting the JA--fluoresceine conjugate, exhibited significant biological activities in a rice seedling assay, a rice phytoalexin-inducing assay, and/or a soybean phenylalanine ammonia-lyase-inducing assay. These JA derivatives could therefore be useful probes for identifying JA--binding proteins. The activity spectra of the prepared compounds were different from each other, suggesting that different types of JA receptors were involved in the perception of JA derivatives in the respective bioassays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / chemistry*
  • Biotin / analogs & derivatives*
  • Biotin / chemistry
  • Cyclopentanes / chemical synthesis
  • Cyclopentanes / chemistry
  • Cyclopentanes / metabolism*
  • Dexamethasone / analogs & derivatives*
  • Dexamethasone / chemistry
  • Fluoresceins / chemistry*
  • Molecular Probes / chemical synthesis*
  • Molecular Probes / metabolism
  • Oryza / metabolism
  • Oxylipins
  • Phenylalanine Ammonia-Lyase / metabolism
  • Plant Extracts / metabolism
  • Protein Binding
  • Sesquiterpenes
  • Soybeans / metabolism
  • Terpenes

Substances

  • Amino Acids
  • Cyclopentanes
  • Fluoresceins
  • Molecular Probes
  • Oxylipins
  • Plant Extracts
  • Sesquiterpenes
  • Terpenes
  • phytoalexins
  • jasmonic acid
  • Biotin
  • Dexamethasone
  • Phenylalanine Ammonia-Lyase