Immunoassays for collagens in chondrocyte and cartilage explant cultures

R Clark Billinghurst; Fackson Mwale; Anthony Hollander; Mirela Ionescu; A Robin Poole

doi:10.1385/1-59259-810-2:251

Immunoassays for collagens in chondrocyte and cartilage explant cultures

Methods Mol Med. 2004:100:251-74. doi: 10.1385/1-59259-810-2:251.

Authors

R Clark Billinghurst¹, Fackson Mwale, Anthony Hollander, Mirela Ionescu, A Robin Poole

Affiliation

¹ Department of Health Sciences, St. Lawrence College, Kingston, Ontario, Canada.

PMID: 15280600
DOI: 10.1385/1-59259-810-2:251

Abstract

Quantitative immunoassays have been developed to measure the content, degradation, and synthesis of types II and IX collagens in hyaline cartilages. Some of these assays and their applications are described in this chapter. These and other assays are commercially available. The applications of these assays are discussed with examples from recent publications.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Antibodies / immunology
Cartilage / chemistry*
Cartilage / immunology
Cells, Cultured
Chondrocytes / chemistry*
Chondrocytes / immunology
Collagen Type II / analysis*
Collagen Type II / immunology*
Collagen Type II / metabolism
Collagen Type X / analysis*
Collagen Type X / immunology*
Collagen Type X / metabolism
Enzyme-Linked Immunosorbent Assay / methods*
Humans
Molecular Sequence Data

Substances

Antibodies
Collagen Type II
Collagen Type X