Pax7 directs postnatal renewal and propagation of myogenic satellite cells but not their specification

EMBO J. 2004 Aug 18;23(16):3430-9. doi: 10.1038/sj.emboj.7600346. Epub 2004 Jul 29.

Abstract

The paired-box transcription factor Pax7 has been claimed to specify the muscle stem cell lineage since inactivation of Pax7 led to a failure to detect muscle satellite cells. Here we show that muscles of juvenile Pax7(-/-) mice at P11 contain a reduced but substantial number of satellite cells. Neither juvenile nor adult Pax7(-/-) mice displayed a significant reduction in the number and size of myotubes, indicating that the remaining number of satellite cells sufficed to allow normal postnatal muscle growth. The number of satellite cells in Pax7 mutant mice declined strongly during postnatal development, although single satellite cells were readily identified in adult Pax7 mutant mice. Muscle regeneration was impaired in adult Pax7 mutant mice. Our results clearly indicate an essential function of Pax7 for renewal and maintenance of muscle stem cells and exclude an exclusive role of Pax7 in satellite cell specification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / physiology*
  • Animals
  • Cell Differentiation*
  • Cell Proliferation
  • Cells, Cultured
  • DNA-Binding Proteins / metabolism
  • Gene Deletion
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism*
  • Mice
  • Microscopy, Electron
  • Muscle Development*
  • Muscle Proteins / metabolism
  • Mutation / genetics
  • MyoD Protein / metabolism
  • Myogenic Regulatory Factor 5
  • PAX3 Transcription Factor
  • PAX7 Transcription Factor
  • Paired Box Transcription Factors
  • Regeneration
  • Satellite Cells, Skeletal Muscle / cytology*
  • Satellite Cells, Skeletal Muscle / metabolism*
  • Trans-Activators / metabolism
  • Transcription Factors / metabolism
  • Up-Regulation

Substances

  • DNA-Binding Proteins
  • Homeodomain Proteins
  • Muscle Proteins
  • Myf5 protein, mouse
  • MyoD Protein
  • Myogenic Regulatory Factor 5
  • PAX3 Transcription Factor
  • PAX7 Transcription Factor
  • Paired Box Transcription Factors
  • Pax7 protein, mouse
  • Trans-Activators
  • Transcription Factors
  • Pax3 protein, mouse