Thyrocyte-specific expression of Cre recombinase in transgenic mice

Genesis. 2004 Jul;39(3):212-6. doi: 10.1002/gene.20043.


A transgenic mouse line that expresses Cre recombinase under control of the human thyroid peroxidase (TPO) gene promoter was established. The activity and specificity of the TPO-driven Cre recombinase were examined by using Northern blotting and by crossing with the ROSA26 reporter transgenic mouse line. In the latter mice, Cre-mediated recombination occurred only in the thyrocytes, and recombination commenced around embryonic day 14.5, at the time during thyroid organogenesis when TPO expression begins. This study demonstrates that the TPO-Cre transgenic mouse is a powerful tool to specifically delete loxP-inserted (floxed) genes in thyrocytes and will be of great value in the study of thyrocyte-specific genes during development and/or in adult thyroids.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Autoantigens / genetics
  • Blotting, Northern
  • Crosses, Genetic
  • Galactosides
  • Gene Targeting / methods*
  • Immunohistochemistry
  • Indoles
  • Integrases / metabolism*
  • Iodide Peroxidase / genetics
  • Iron-Binding Proteins / genetics
  • Mice
  • Mice, Transgenic
  • Organogenesis
  • Promoter Regions, Genetic / genetics
  • Recombination, Genetic / genetics*
  • Thyroid Gland / cytology
  • Thyroid Gland / embryology
  • Thyroid Gland / metabolism*


  • Autoantigens
  • Galactosides
  • Indoles
  • Iron-Binding Proteins
  • TPO protein, human
  • Iodide Peroxidase
  • Cre recombinase
  • Integrases
  • 5-bromo-4-chloro-3-indolyl beta-galactoside