Copper deficiency induced expression of Fe-superoxide dismutase gene in Matteuccia struthiopteris

Plant Physiol Biochem. 2004 Feb;42(2):143-8. doi: 10.1016/j.plaphy.2003.11.004.

Abstract

Iron-superoxide dismutase (Fe-SOD) activity was not detected in extracts from the leaves of ferns, Equisetum arvense and Matteuccia struthiopteris. To know why ferns lack Fe-SOD activity, the Fe-SOD like gene (MsFeSOD1) was isolated from M. struthiopteris and its expression was investigated with a focus on the metals Fe and Cu using the prothalli of the fern. The expression of MsFeSOD1 mRNA was induced by a deficiency of Cu, but Fe-SOD activity was not detected. The recombinant protein of MsFeSOD1 produced in E. coli showed Fe-SOD activity. These findings suggest that the fern Fe-SOD like gene was transcriptionally regulated by Cu but an additional mechanism is involved in the formation of an active enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Conserved Sequence
  • Copper / deficiency
  • Copper / pharmacology
  • Copper / physiology*
  • Ferns / enzymology*
  • Ferns / genetics*
  • Gene Expression Regulation, Enzymologic / drug effects
  • Gene Expression Regulation, Plant / drug effects*
  • Molecular Sequence Data
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Superoxide Dismutase / chemistry
  • Superoxide Dismutase / genetics*

Substances

  • Recombinant Proteins
  • Copper
  • Superoxide Dismutase