Interactions of butyric acid- and acetic acid-treated Salmonella with chicken primary cecal epithelial cells in vitro

Avian Dis. 2004 Apr-Jun;48(2):384-91. doi: 10.1637/7094.

Abstract

In vitro studies of the interaction between pathogenic bacteria and the chicken intestinal epithelium are hampered by the lack of a host- and tissue-specific in vitro model. Therefore, a reproducible method for isolation and cultivation of chicken primary cecal epithelial cells was developed. Cecal crypts were isolated and cultured in vitro to form a semiconfluent layer of epithelial cells. Incubation of Salmonella enteritidis with these cells resulted in invasion. Pretreatment of the Salmonella bacteria with butyric acid resulted in a significant decrease of invasion of the bacteria in the chicken cecal epithelial cells, whereas pretreatment with acetic acid increased invasiveness. These interactions of S. enteritidis with primary chicken cecal epithelial cells were similar to the interactions with other epithelial cell types.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetic Acid / pharmacology*
  • Animals
  • Butyric Acid / pharmacology*
  • Cecum / microbiology*
  • Cells, Cultured
  • Chickens
  • Epithelial Cells / metabolism
  • Epithelial Cells / microbiology*
  • Epithelial Cells / ultrastructure
  • Female
  • Immunohistochemistry / veterinary
  • Intestinal Mucosa / metabolism
  • Intestinal Mucosa / microbiology*
  • Intestinal Mucosa / ultrastructure
  • Keratins / metabolism
  • Microscopy, Electron, Scanning
  • Salmonella enteritidis / drug effects
  • Salmonella enteritidis / metabolism
  • Salmonella enteritidis / physiology*
  • Vimentin / metabolism

Substances

  • Vimentin
  • Butyric Acid
  • Keratins
  • Acetic Acid