A single, multiplex analysis for all relevant activating NRAS gene mutations using heteroduplex generators

Br J Haematol. 2004 Aug;126(4):602-5. doi: 10.1111/j.1365-2141.2004.05079.x.


We describe a multiplex polymerase chain reaction (PCR)-based test that detected all relevant NRAS activating mutations using a single PCR followed directly by electrophoresis. The test uses a Universal Heteroduplex Generator (UHG) to detect exon-2 (codon 61) NRAS mutations in multiplex with an UHG for exon-1 (codons 12 and 13). The method differentiated all 19 relevant mutations in these exons and showed a mutation independent sensitivity of approximately 6%. The sensitive, specific detection of all NRAS activating mutations using this single rapid test represents a minimum workload and could be applied readily for large-scale screening and for routine analysis.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Genes, ras / genetics*
  • Heteroduplex Analysis / methods*
  • Humans
  • Molecular Sequence Data
  • Mutation*
  • Polymerase Chain Reaction / methods
  • Sensitivity and Specificity