Chemotaxis of Pseudomonas stutzeri OX1 and Burkholderia cepacia G4 toward chlorinated ethenes

Appl Microbiol Biotechnol. 2005 Mar;66(6):696-701. doi: 10.1007/s00253-004-1685-4. Epub 2004 Jul 28.


The chemotactic responses of Pseudomonas putida F1, Burkholderia cepacia G4, and Pseudomonas stutzeri OX1 were investigated toward toluene, trichloroethylene (TCE), tetrachloroethylene (PCE), cis-1,2-dichloroethylene (cis-DCE), trans-1,2-dichloroethylene (trans-DCE), 1,1-dichloroethylene (1,1-DCE), and vinyl chloride (VC). P. stutzeri OX1 and P. putida F1 were chemotactic toward toluene, PCE, TCE, all DCEs, and VC. B. cepacia G4 was chemotactic toward toluene, PCE, TCE, cis-DCE, 1,1-DCE, and VC. Chemotaxis of P. stutzeri OX1 grown on o-xylene vapors was much stronger than when grown on o-cresol vapors toward some chlorinated ethenes. Expression of toluene-o-xylene monooxygenase (ToMO) from touABCDEF appears to be required for positive chemotaxis attraction, and the attraction is stronger with the touR (ToMO regulatory) gene.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adaptation, Physiological
  • Burkholderia cepacia / genetics
  • Burkholderia cepacia / physiology*
  • Chemotaxis / physiology*
  • Gene Expression Regulation, Bacterial
  • Genes, Regulator
  • Hydrocarbons, Chlorinated / metabolism*
  • Microscopy
  • Oxygenases / genetics
  • Oxygenases / physiology
  • Pseudomonas putida / genetics
  • Pseudomonas putida / physiology
  • Pseudomonas stutzeri / genetics
  • Pseudomonas stutzeri / physiology*


  • Hydrocarbons, Chlorinated
  • Oxygenases
  • toluene 2-xylene monooxygenase