Cre/loxP system for generating tissue-specific knockout mouse models

Nutr Rev. 2004 Jun;62(6 Pt 1):243-6. doi: 10.1301/nr2004.jun243-246.


Alteration of the mouse genome by conventional transgenic and gene-targeted approaches has greatly facilitated studies of gene function. However, a gene alteration expressed in the germ line may cause an embryonic lethal phenotype resulting in no viable mouse to study gene function. Similarly, a gene alteration may exert its effect in multiple different cell and tissue types, creating a complex phenotype in which it is difficult to distinguish direct function in a particular tissue from secondary effects resulting from altered gene function in other tissues. Therefore, methods have been developed to control conditions such as the timing, cell-type, and tissue specificity of gene activation or repression. This brief review provides an overview of the Cre/LoxP system for generating tissue-specific knockout mouse models.

Publication types

  • Review

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Integrases
  • Mice
  • Mice, Knockout*
  • Models, Animal*
  • Nutritional Physiological Phenomena*
  • Viral Proteins


  • Viral Proteins
  • Cre recombinase
  • Integrases