Characterization of two Bacillus thuringiensis genes identified by in vivo screening of virulence factors

Appl Environ Microbiol. 2004 Aug;70(8):4784-91. doi: 10.1128/AEM.70.8.4784-4791.2004.

Abstract

Bacillus thuringiensis vegetative cells are known to be highly pathogenic when injected into the hemocoel of susceptible insect larvae. This pathogenicity is due to the capacity of B. thuringiensis to cause septicemia in the host. We screened a B. thuringiensis mini-Tn10 insertion library for loss of virulence against Bombyx mori larvae on injection into the hemocoel. Three clones with attenuated virulence were isolated, corresponding to two different mini-Tn10 insertions mapping to the yqgB/yqfZ locus. Single disruptions of the yqgB and yqfZ genes did not affect virulence against B. mori. In contrast, the inactivation of both genes simultaneously reproduced the effect of the mini-Tn10 insertion and resulted in a significant delay to infection. The double DeltayqgB DeltayqfZ mutant was also nonmotile, and its growth was affected at 25 degrees C. We analyzed lacZ transcriptional fusions and detected promoter activity upstream from yqgB at 25 and 37 degrees C. Overall, our findings suggest that the yqgB and yqfZ genes encode adaptive factors that may act in synergy, enabling the bacteria to cope with the physical environment in vivo, facilitating colonization of the host.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacillus thuringiensis / genetics
  • Bacillus thuringiensis / growth & development
  • Bacillus thuringiensis / pathogenicity*
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Bombyx / microbiology
  • DNA Transposable Elements
  • Gene Expression Regulation, Bacterial*
  • Larva / microbiology
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Mutagenesis, Site-Directed
  • Sequence Analysis, DNA
  • Virulence
  • Virulence Factors / genetics*
  • Virulence Factors / metabolism

Substances

  • Bacterial Proteins
  • DNA Transposable Elements
  • Virulence Factors

Associated data

  • GENBANK/AY455944