Constant-infusion H(2)15O PET and acetazolamide challenge in the assessment of cerebral perfusion status

J Nucl Med. 2004 Aug;45(8):1344-50.


Assessing the baseline perfusion and perfusion reserve after acetazolamide (ACZ) challenge is a common method for the evaluation of patients with cerebrovascular disease. Most previous studies using H(2)(15)O PET applied the bolus injection technique. There is considerable discrepancy regarding the optimal time point of imaging after ACZ injection. The purpose of this study was to continuously monitor cerebral blood flow (CBF) after ACZ using constant-infusion H(2)(15)O PET.

Methods: Four patients with stenoses of an internal carotid artery and 6 with moyamoya disease were studied. H(2)(15)O was continuously infused, and data were recorded in 1-min frames. After equilibration of H(2)(15)O, 5 min of baseline data were acquired, and then 1 g of ACZ was administered intravenously and data collection continued for 10-22 min. Arterial blood was continuously drawn for absolute quantification of CBF.

Results: The arterial (15)O concentration remained generally stable during scanning, and the cerebellar blood flow fluctuations of the 5 baseline scans were small. The scan-to-scan difference was 6% (difference of 2 successive scans/mean). In the nonpathologic areas, the increase in CBF started 1-2 min after administration of ACZ. The largest fraction of the increase occurred from 0 to 10 min. The ratio of CBF in pathologic areas to CBF in cerebellum showed an initial decrease that stabilized after 5 min.

Conclusion: A continuous-infusion protocol is a viable alternative to single bolus injections for the assessment of cerebral perfusion status. Such a protocol is advantageous when the time course of CBF after an intervention is not known. With continuous monitoring, the optimal time point for evaluation of a certain parameter can be chosen post hoc. Furthermore, the time course of CBF itself may allow the definition of new parameters for evaluating perfusion status in cerebrovascular patients, both for assessment before a revascularization procedure and for follow-up. A limitation of the present study is the relatively small number of patients with each type of cerebrovascular disease and the lack of healthy subjects.

Publication types

  • Clinical Trial
  • Comparative Study
  • Controlled Clinical Trial
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Acetazolamide*
  • Adolescent
  • Adult
  • Aged
  • Anticonvulsants
  • Brain / blood supply*
  • Brain / diagnostic imaging*
  • Brain / drug effects
  • Carotid Stenosis / diagnostic imaging*
  • Cerebrovascular Circulation*
  • Child
  • Humans
  • Infusions, Intravenous / methods
  • Male
  • Middle Aged
  • Moyamoya Disease / diagnostic imaging*
  • Oxygen Radioisotopes* / administration & dosage
  • Severity of Illness Index
  • Tomography, Emission-Computed / methods
  • Water


  • Anticonvulsants
  • Oxygen Radioisotopes
  • Water
  • Acetazolamide