Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines

World J Gastroenterol. 2004 Sep 15;10(18):2643-6. doi: 10.3748/wjg.v10.i18.2643.


Aim: To investigate the effect of histone acetylation on regulation of p21WAF1 gene expression in human colon cancer cell lines.

Methods: Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate. Expressions of p21WAF1 mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively. Acetylation of two regions of p21WAF1 gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay and Western blotting.

Results: Trichostatin or sodium butyrate re-activated p21WAF1 transcription resulted in up-regulated p21WAF1 protein level in colon cancer cell lines. Those effects were accompanied by an accumulation of acetylated histones in total cellular chromatin and p21WAF1 gene-associated region of chromatin.

Conclusion: Histone acetylation regulates p21WAF1 expression in human colon cancer cell lines, Colo-320 and SW1116.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Butyrates / pharmacology
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism
  • Cell Line, Tumor
  • Colonic Neoplasms*
  • Cyclin-Dependent Kinase Inhibitor p21
  • Gene Expression Regulation, Neoplastic / drug effects
  • Gene Expression Regulation, Neoplastic / physiology*
  • Histones / metabolism*
  • Humans
  • Hydroxamic Acids / pharmacology
  • Protein Synthesis Inhibitors / pharmacology
  • RNA, Messenger / analysis


  • Butyrates
  • CDKN1A protein, human
  • Cell Cycle Proteins
  • Cyclin-Dependent Kinase Inhibitor p21
  • Histones
  • Hydroxamic Acids
  • Protein Synthesis Inhibitors
  • RNA, Messenger
  • trichostatin A