[cDNA microarray analysis of gene expression in acquired methotrexate-resistant of human choriocarcinoma]

Zhonghua Fu Chan Ke Za Zhi. 2004 Jun;39(6):396-9.
[Article in Chinese]


Objective: To identify the molecular components involved in acquired methotrexate-resistance of human choriocarcinoma.

Methods: Methotrexate-resistant cell line (JAR/MTX) was derived from JAR cell line by exposed to intervally and progressively increasing higher concentration of MTX. cDNA microarray analyses of JAR/MTX and its parental cell line JAR were performed.

Results: JAR/MTX was established after one year with stable resistance. Its resistant index to MTX was 7.3. Nine genes were differential expressed between JAR/MTX and JAR cells. INSR, SLC1A3, SAT, HBB, and FLJ12443 were underexpressed and HS1, TXNRD1, TAGLN2, and EEF2 were overexpressed in JAR/MTX cells.

Conclusion: The cDNA microarray system showed that several alterations of gene expression were present in acquired methotrexate-resistance of human choriocarcinoma.

Publication types

  • English Abstract

MeSH terms

  • Apoptosis
  • Cell Adhesion
  • Cell Division
  • Cell Line, Tumor
  • Choriocarcinoma / drug therapy
  • Choriocarcinoma / genetics
  • Choriocarcinoma / pathology*
  • DNA, Complementary / analysis
  • Drug Resistance, Neoplasm / genetics*
  • Female
  • Gene Expression Profiling* / methods
  • Humans
  • Methotrexate / pharmacology*
  • Oligonucleotide Array Sequence Analysis


  • DNA, Complementary
  • Methotrexate