Blockade of Transgenic Gamma Delta T Cell Development in Beta 2-microglobulin Deficient Mice

EMBO J. 1992 Jan;11(1):25-31.

Abstract

The gamma delta T cell receptor (TCR) of the hybridoma KN6 recognizes the self molecule encoded by a class I gene which maps within the TL region of the major histocompatibility complex (MHC) of H-2b mice. Mice transgenic (Tg) for this TCR were crossed with mice genetically deficient in beta 2-microglobulin (beta 2m). No mature Tg gamma delta T cells were detected in the thymus or the spleen of the beta 2m- gamma delta Tg mice. We conclude that interaction between the Tg gamma delta TCR and a beta 2m-associated molecule (probably an MHC class I molecule) is required for the generation of mature Tg gamma delta T cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Antibodies, Monoclonal / immunology
  • Antigens, CD*
  • Antigens, Differentiation / immunology
  • Base Sequence
  • CD24 Antigen
  • Cell Differentiation*
  • Crosses, Genetic
  • Embryo, Mammalian / immunology
  • Gene Expression
  • Genotype
  • Haplotypes
  • Immunohistochemistry
  • Membrane Glycoproteins*
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Receptors, Antigen, T-Cell, gamma-delta / genetics*
  • Receptors, Antigen, T-Cell, gamma-delta / metabolism
  • Spleen / cytology
  • T-Lymphocytes / metabolism*
  • Thymus Gland / cytology
  • Thymus Gland / embryology
  • beta 2-Microglobulin / deficiency
  • beta 2-Microglobulin / genetics*

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Differentiation
  • CD24 Antigen
  • Cd24a protein, mouse
  • Membrane Glycoproteins
  • Receptors, Antigen, T-Cell, gamma-delta
  • beta 2-Microglobulin