Abstract
Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone to produce 9-fluorenone and 2'-carboxy-2,3-dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded by flnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinomycetales / genetics*
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Actinomycetales / metabolism*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Bacterial Proteins / physiology
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Biodegradation, Environmental
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Biphenyl Compounds / metabolism
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Catechols / metabolism
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DNA, Bacterial / chemistry
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DNA, Bacterial / isolation & purification
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Fluorenes / metabolism*
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Gene Order
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Genes, Bacterial* / genetics
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Genes, Bacterial* / physiology
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Hydrolases / genetics
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Hydrolases / metabolism
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Mixed Function Oxygenases / genetics
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Mixed Function Oxygenases / metabolism
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Molecular Sequence Data
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Oxidoreductases / genetics
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Oxidoreductases / metabolism
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Oxygenases / genetics
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Oxygenases / metabolism
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Phthalic Acids / metabolism
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Sequence Analysis, DNA
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Sequence Homology
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Synteny
Substances
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Bacterial Proteins
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Biphenyl Compounds
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Catechols
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DNA, Bacterial
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Fluorenes
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Phthalic Acids
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2,3-dihydroxybiphenyl
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1,1a-dihydroxy-1-hydrofluoren-9-one
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fluorene
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phthalic acid
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9-fluorenone
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Mixed Function Oxygenases
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Oxidoreductases
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Oxygenases
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extradiol dioxygenase
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Hydrolases