Automated NMR structure calculation with CYANA

Methods Mol Biol. 2004;278:353-78. doi: 10.1385/1-59259-809-9:353.

Abstract

This chapter gives an introduction to automated nuclear magnetic resonance (NMR) structure calculation with the program CYANA. Given a sufficiently complete list of assigned chemical shifts and one or several lists of cross-peak positions and columns from two-, three-, or four-dimensional nuclear Overhauser effect spectroscopy (NOESY) spectra, the assignment of the NOESY cross-peaks and the three-dimensional structure of the protein in solution can be calculated automatically with CYANA.

MeSH terms

  • Automation
  • Hemeproteins / chemistry
  • Models, Molecular
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Protein Conformation*
  • Proteins / chemistry*
  • Software*

Substances

  • Hemeproteins
  • Proteins