TCR gamma delta bearing T lymphocytes infiltrating human primary cutaneous melanomas

J Invest Dermatol. 1992 Mar;98(3):369-74. doi: 10.1111/1523-1747.ep12499808.


In situ immunophenotypic studies show that tumor infiltrating T lymphocytes from six of 11 fresh cutaneous primary melanomas tested contain T-cell receptor gamma delta expressing cells whereas those from 8 metastatic melanomas did not. In three cases these cells represent an unexpectedly high proportion of the lymphocytic infiltrate, ranging from 15 to 25%. This elevated percentage is maintained when tumor infiltrating lymphocytes are propagated in vitro for 2 or 3 weeks in the presence of recombinant interleukin 2 and autologous tumor cells. Functional studies reveal that gamma delta lines or clones developed from lymphocytes infiltrating primary melanoma display, in addition to their natural killer-like activity, a potent autologous tumor cell cytotoxic activity that does not seem to be restricted by major histocompatibility complex gene products.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Lymphocytes, Tumor-Infiltrating / immunology*
  • Melanoma / immunology*
  • Neoplasm Metastasis
  • Phenotype
  • Receptors, Antigen, T-Cell, alpha-beta / analysis
  • Receptors, Antigen, T-Cell, gamma-delta / analysis*
  • Skin Neoplasms / immunology*
  • T-Lymphocytes / immunology*
  • Tumor Cells, Cultured


  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Antigen, T-Cell, gamma-delta