Development of a PCR-based mating-type assay for Clavicipitaceae

FEMS Microbiol Lett. 2004 Aug 15;237(2):205-12. doi: 10.1016/j.femsle.2004.06.033.

Abstract

This study developed a PCR-based mating-type assay for Clavicipitaceae. PCR primer sets for the mating-type genes MAT1-1-1 and MAT1-2-1 were designed based on the amino acid sequences of the conserved alpha and HMG boxes, respectively. The PCR-based mating-type assay could be applied for various clavicipitaceous genera (Balansia, Claviceps, Cordyceps and Epichloë). Most of the clavicipitaceous fungi possessed either MAT1-1-1 or MAT1-2-1, and were supposed to be heterothallic. Although the PCR products obtained by the mating-type assay were short, the phylogenetic trees of the mating-type genes gave better resolutions than that of 18S rDNA and agreed well to their econutritional modes.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers
  • DNA, Fungal / classification
  • Fungal Proteins / classification
  • Fungal Proteins / genetics
  • Genes, Fungal*
  • Genes, Mating Type, Fungal*
  • Genotype
  • Hypocreales / classification
  • Hypocreales / genetics*
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction / methods*
  • RNA, Ribosomal, 18S / genetics

Substances

  • DNA Primers
  • DNA, Fungal
  • Fungal Proteins
  • RNA, Ribosomal, 18S

Associated data

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