The generation of interferon-gamma-producing T lymphocytes in skin-draining lymph nodes, and their recruitment to the lungs, is associated with protective immunity to Schistosoma mansoni

Immunology. 1992 Feb;75(2):250-6.


We have examined immunological responses in the skin-draining lymph nodes (SLN) and lungs of mice during the 3 weeks after percutaneous exposure to attenuated larvae of Schistosoma mansoni. Cercariae irradiated with 20 krads (V20) were highly protective and sustained an increased number of CD4+ T cells in the SLN. On secondary exposure to schistosome antigen in vitro, these cells were capable of proliferating and secreting high levels of interferon-gamma (IFN-gamma) and interleukin-3 (IL-3). However, in mice exposed to non-protective 80 krad-irradiated (V80) cercariae, secretion of these cytokines occurred early and only transiently. Significantly elevated numbers of CD4+ T lymphocytes were recoverable on Day 21 from the lungs of V20, but not V80 mice. These cells secreted high levels of IFN-gamma and IL-3 in vitro, but not IL-2 and IL-4. Mice immunized intravenously with attenuated lung-stage schistosomula were not protected, despite having an elevated pulmonary lymphocyte population. Moreover these cells failed to secrete IFN-gamma and IL-3. However, significant protection was achieved where exposure of mice to a combination of V80 cercariae and lung-stage schistosomula resulted in the recruitment of IFN-gamma secreting cells to the lungs. We conclude that the success of the irradiated vaccine depends not only on the generation of a population of antigen-specific T-helper cells in the SLN, but also recruitment of these cells to the lungs before challenge.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Helminth / immunology
  • Bronchoalveolar Lavage Fluid / immunology
  • Cells, Cultured
  • Female
  • Interferon-gamma / biosynthesis*
  • Lung / immunology*
  • Lymph Nodes / immunology*
  • Mediastinum
  • Mice
  • Mice, Inbred C57BL
  • Schistosoma mansoni / immunology
  • Schistosomiasis mansoni / immunology*
  • Schistosomiasis mansoni / prevention & control
  • Skin
  • T-Lymphocytes, Helper-Inducer / immunology*


  • Antigens, Helminth
  • Interferon-gamma