Drosophila cohesins DSA1 and Drad21 persist and colocalize along the centromeric heterochromatin during mitosis

Biol Cell. 2004 Aug;96(6):457-62. doi: 10.1016/j.biolcel.2004.04.011.

Abstract

Sister chromatid cohesion in eukaryotes is maintained mainly by a conserved multiprotein complex termed cohesin. Drad21 and DSA1 are the Drosophila homologues of the yeast Scc1 and Scc3 cohesin subunits, respectively. We recently identified a Drosophila mitotic cohesin complex composed of Drad21/DSA1/DSMC1/DSMC3. Here we study the contribution of this complex to sister chromatid cohesion using immunofluorescence microscopy to analyze cell cycle chromosomal localization of DSA1 and Drad21 in S2 cells. We observed that DSA1 and Drad21 colocalize during all cell cycle stages in cultured cells. Both proteins remain in the centromere until metaphase, colocalizing at the centromere pairing domain that extends along the entire heterochromatin; the centromeric cohesion protein MEI-S332 is nonetheless reported in a distinct centromere domain. These results provide strong evidence that DSA1 and Drad21 are partners in a cohesin complex involved in the maintenance of sister chromatid arm and centromeric cohesion during mitosis in Drosophila.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle Proteins / metabolism*
  • Cells, Cultured
  • Centromere / metabolism*
  • Chromosomal Proteins, Non-Histone
  • Chromosomes
  • Drosophila / metabolism*
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / physiology*
  • Fungal Proteins / metabolism*
  • Heterochromatin / metabolism*
  • Humans
  • Mitosis / physiology*
  • Nuclear Proteins / metabolism*
  • Protein Subunits / metabolism

Substances

  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • Drosophila Proteins
  • Fungal Proteins
  • Heterochromatin
  • Nuclear Proteins
  • Protein Subunits
  • SA protein, Drosophila
  • cohesins
  • vtd protein, Drosophila