Sexually dimorphic expression of a teleost homologue of Müllerian inhibiting substance during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus

Biochem Biophys Res Commun. 2004 Sep 17;322(2):508-13. doi: 10.1016/j.bbrc.2004.07.162.


Müllerian inhibiting substance (MIS), also known as anti-Müllerian hormone, is a glycoprotein belonging to transforming growth factor beta superfamily. In mammals, MIS is responsible for regression of Müllerian ducts, anlagen of the female reproductive ducts, in the male fetus. However, the role of MIS in gonadal sex differentiation of teleost fishes, which do not have the Müllerian ducts, has yet to be clarified. To address the role of MIS on gonadal sex differentiation in fishes, we isolated a MIS cDNA from the Japanese flounder testis and examined the expression pattern of MIS mRNA in gonads of both sexes during sex differentiation period. In this study, we present the first demonstration of sexually dimorphic expression of MIS mRNA during sex differentiation in teleost fishes, similarly to amniote vertebrates which possess the Müllerian ducts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Mullerian Hormone
  • Aromatase / genetics
  • Aromatase / metabolism
  • Chick Embryo
  • Female
  • Flounder / metabolism*
  • Glycoproteins / genetics
  • Glycoproteins / metabolism*
  • Humans
  • In Situ Hybridization
  • Male
  • Mice
  • Molecular Sequence Data
  • Ovary / embryology*
  • Ovary / metabolism
  • RNA, Messenger / metabolism
  • Testicular Hormones / genetics
  • Testicular Hormones / metabolism*
  • Testis / embryology*
  • Testis / metabolism


  • Glycoproteins
  • RNA, Messenger
  • Testicular Hormones
  • Anti-Mullerian Hormone
  • Aromatase