Interleukin-10 in combination with M-CSF and IL-4 contributes to development of the rare population of CD14+CD16++ cells derived from human monocytes

Biochem Biophys Res Commun. 2004 Sep 17;322(2):637-43. doi: 10.1016/j.bbrc.2004.07.172.


Peripheral blood CD14(+)CD16(++) monocytes (Mo) are a rare Mo subpopulation known to undergo expansion in various diseases. We show here that IL-10 in the presence of M-CSF and IL-4 triggers the generation of CD14(+)CD16(++) cells from highly purified human cord blood (CB) and adult blood Mo. CB Mo were more sensitive to this cytokine combination than adult Mo. IL-10-induced CD14(+)CD16(++) cells that expressed dendritic cell markers: CD80, CD86, HLA-DR, and CD83 and initiated significantly decreased allogeneic mixed lymphocyte reactions (MLRs). Blockage of CD86, but not CD80, further down-modulated MLRs induced by CD14(+)CD16(++)cells. CD14(+)CD16(++) cells had similar features to CD14(+)CD16(++) Mo in that they expressed increased level of CCR5, efficiently produced TNF-alpha, and displayed higher MLR than CD14(+)CD16(-) Mo. Together, these results demonstrate that M-CSF, IL-4, and IL-10 drive Mo into CD14(+)CD16(++) cells similar to those identified in vivo, and CB Mo, due to their increased responsiveness, may be a useful starting cell source to study differentiation of CD14(+)CD16(++) cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Dendritic Cells / metabolism
  • Fetal Blood
  • Humans
  • Interleukin-10 / metabolism*
  • Interleukin-4 / metabolism*
  • Lipopolysaccharide Receptors / metabolism*
  • Macrophage Colony-Stimulating Factor / metabolism*
  • Monocytes / metabolism*
  • Receptors, CCR5 / metabolism
  • Receptors, IgG / metabolism*
  • Tumor Necrosis Factor-alpha / metabolism


  • Lipopolysaccharide Receptors
  • Receptors, CCR5
  • Receptors, IgG
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Interleukin-4
  • Macrophage Colony-Stimulating Factor