Simulations of action of DNA topoisomerases to investigate boundaries and shapes of spaces of knots

Biophys J. 2004 Nov;87(5):2968-75. doi: 10.1529/biophysj.104.045864. Epub 2004 Aug 23.

Abstract

The configuration space available to randomly cyclized polymers is divided into subspaces accessible to individual knot types. A phantom chain utilized in numerical simulations of polymers can explore all subspaces, whereas a real closed chain forming a figure-of-eight knot, for example, is confined to a subspace corresponding to this knot type only. One can conceptually compare the assembly of configuration spaces of various knot types to a complex foam where individual cells delimit the configuration space available to a given knot type. Neighboring cells in the foam harbor knots that can be converted into each other by just one intersegmental passage. Such a segment-segment passage occurring at the level of knotted configurations corresponds to a passage through the interface between neighboring cells in the foamy knot space. Using a DNA topoisomerase-inspired simulation approach we characterize here the effective interface area between neighboring knot spaces as well as the surface-to-volume ratio of individual knot spaces. These results provide a reference system required for better understanding mechanisms of action of various DNA topoisomerases.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage T4 / genetics*
  • Computer Simulation
  • DNA, Viral / chemistry*
  • Models, Chemical*
  • Models, Molecular*
  • Models, Statistical
  • Nucleic Acid Conformation

Substances

  • DNA, Viral