Abstract
Lv1 restriction of HIV-1 in the cells of Old World monkeys is associated with the expression of the Trim5 gene. Uniquely, in owl monkey kidney cells, HIV-1 restriction is dependent on the ability of incoming viral capsid protein to bind cyclophilin A (CypA). Cloning of the owl monkey Trim5 gene now reveals the presence of an inserted CypA pseudogene within intron 7 of the Trim5 gene. This insertion results in the formation of a chimeric Trim5-CypA transcript. Transfer of a cDNA corresponding to this transcript into human cells confers cyclosporin A-sensitive resistance to HIV-1 infection. The restriction factor appears to be a chimeric protein created by retrotransposon-mediated exon shuffling.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acquired Immunodeficiency Syndrome / prevention & control*
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Amino Acid Sequence
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Animals
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Aotidae / genetics*
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Base Sequence
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Capsid Proteins / metabolism
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Chromosome Mapping
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Cyclophilin A / genetics*
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HIV-1 / growth & development*
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Kidney / metabolism*
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Molecular Sequence Data
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Proteins / genetics*
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RNA, Messenger / analysis
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Recombinant Fusion Proteins / genetics*
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Ubiquitin-Protein Ligases
Substances
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Capsid Proteins
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Proteins
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RNA, Messenger
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Recombinant Fusion Proteins
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TRIM5(alpha) protein, rhesus monkey
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Ubiquitin-Protein Ligases
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Cyclophilin A
Associated data
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GENBANK/AY684991
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GENBANK/AY684992
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GENBANK/AY684993
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GENBANK/AY684994
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GENBANK/AY684995
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GENBANK/AY684996
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GENBANK/AY684997