A Trim5-cyclophilin A fusion protein found in owl monkey kidney cells can restrict HIV-1

Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13324-8. doi: 10.1073/pnas.0404640101. Epub 2004 Aug 23.


Lv1 restriction of HIV-1 in the cells of Old World monkeys is associated with the expression of the Trim5 gene. Uniquely, in owl monkey kidney cells, HIV-1 restriction is dependent on the ability of incoming viral capsid protein to bind cyclophilin A (CypA). Cloning of the owl monkey Trim5 gene now reveals the presence of an inserted CypA pseudogene within intron 7 of the Trim5 gene. This insertion results in the formation of a chimeric Trim5-CypA transcript. Transfer of a cDNA corresponding to this transcript into human cells confers cyclosporin A-sensitive resistance to HIV-1 infection. The restriction factor appears to be a chimeric protein created by retrotransposon-mediated exon shuffling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acquired Immunodeficiency Syndrome / prevention & control*
  • Amino Acid Sequence
  • Animals
  • Aotidae / genetics*
  • Base Sequence
  • Capsid Proteins / metabolism
  • Chromosome Mapping
  • Cyclophilin A / genetics*
  • HIV-1 / growth & development*
  • Kidney / metabolism*
  • Molecular Sequence Data
  • Proteins / genetics*
  • RNA, Messenger / analysis
  • Recombinant Fusion Proteins / genetics*
  • Ubiquitin-Protein Ligases


  • Capsid Proteins
  • Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • TRIM5(alpha) protein, rhesus monkey
  • Ubiquitin-Protein Ligases
  • Cyclophilin A

Associated data

  • GENBANK/AY684991
  • GENBANK/AY684992
  • GENBANK/AY684993
  • GENBANK/AY684994
  • GENBANK/AY684995
  • GENBANK/AY684996
  • GENBANK/AY684997