Phosphate or phosphite addition promotes the proteolytic turnover of phosphate-starvation inducible tomato purple acid phosphatase isozymes

FEBS Lett. 2004 Aug 27;573(1-3):51-4. doi: 10.1016/j.febslet.2004.07.051.

Abstract

Within 48 h of the addition of 2.5 mM phosphate (HPO42-, Pi) or phosphite (H2PO3-, Phi) to 8-day-old Pi-starved (-Pi) tomato suspension cells: (i) secreted and intracellular purple acid phosphatase (PAP) activities decreased by about 12- and 6-fold, respectively and (ii) immunoreactive PAP polypeptides either disappeared (secreted PAPs) or were substantially reduced (intracellular PAP). The degradation of both secreted PAP isozymes was correlated with the de novo synthesis of two extracellular serine proteases having M(r)s of 137 and 121 kDa. In vitro proteolysis of purified secreted tomato PAP isozymes occurred following their 24 h incubation with culture filtrate from Pi-resupplied cells. The results indicate that Pi or Phi addition to -Pi tomato cells induces serine proteases that degrade Pi-starvation inducible extracellular proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase / biosynthesis
  • Acid Phosphatase / metabolism*
  • Blotting, Western
  • Cell Line
  • Endopeptidases / metabolism*
  • Enzyme Induction
  • Glycoproteins / biosynthesis
  • Glycoproteins / metabolism*
  • Isoenzymes / biosynthesis
  • Isoenzymes / metabolism
  • Lycopersicon esculentum / cytology
  • Lycopersicon esculentum / enzymology*
  • Lycopersicon esculentum / metabolism
  • Phosphates / deficiency
  • Phosphates / pharmacology*
  • Phosphites / pharmacology*
  • Time Factors

Substances

  • Glycoproteins
  • Isoenzymes
  • Phosphates
  • Phosphites
  • purple acid phosphatase
  • Acid Phosphatase
  • Endopeptidases