Incidence of Bcl-2 expression in bladder cancer: relation to schistosomiasis

Clin Biochem. 2004 Sep;37(9):798-802. doi: 10.1016/j.clinbiochem.2004.05.017.


Objectives: Apoptosis (programmed cell death) and the genes regulating this process (e.g., Bcl-2) have recently become a focus of interest in the study of cancer development and progression. The bcl-2 gene product plays a role as an inhibitor of apoptosis; it contributes to oncogenesis by suppressing signals that induce apoptotic cell death. The aim of this study was to determine the expression of bcl-2 in schistosomal bladder cancer and to compare it with the established clinicopathological factors.

Methods: Tumor tissues from 118 patients with bladder cancer were examined [57 with squamous cell carcinoma (SCC) and the remaining 61 with transitional cell carcinoma (TCC)]. Of 118 patients, 60 had schistosomiasis associated with bladder cancer. Bcl-2 expression was determined by enzyme immunoassay and the results were confirmed by Western blot and immunodot blot techniques.

Results: Bcl-2 was significantly expressed in SCC compared to those with TCC type in the presence of schistosomiasis. Moreover, bcl-2 was associated with clinical stages and lymph node involvement but not with histological grades.

Conclusions: These observations detect a potential role for bcl-2 expression in schistosomal carcinogenesis, and hence selecting patients for future anti-bcl-2 therapy.

Publication types

  • Comparative Study

MeSH terms

  • Aged
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Transitional Cell / metabolism*
  • Chi-Square Distribution
  • Gene Expression Regulation, Neoplastic / physiology
  • Genes, bcl-2 / physiology*
  • Humans
  • Middle Aged
  • Schistosomiasis / metabolism*
  • Statistics, Nonparametric
  • Urinary Bladder Neoplasms / metabolism*