Simultaneous selected reaction monitoring, MS/MS and MS3 quantitation for the analysis of pharmaceutical compounds in human plasma using chip-based infusion

Luc Alexis Leuthold; Chantal Grivet; Mark Allen; Mark Baumert; Gérard Hopfgartner

doi:10.1002/rcm.1587

Simultaneous selected reaction monitoring, MS/MS and MS3 quantitation for the analysis of pharmaceutical compounds in human plasma using chip-based infusion

Rapid Commun Mass Spectrom. 2004;18(17):1995-2000. doi: 10.1002/rcm.1587.

Authors

Luc Alexis Leuthold¹, Chantal Grivet, Mark Allen, Mark Baumert, Gérard Hopfgartner

Affiliation

¹ Laboratory of Pharmaceutical Analytical Chemistry, Life Sciences Mass Spectrometry, School of Pharmacy, University of Geneva, 20 Boulevard d'Yvoy, CH-1211 Geneva 4, Switzerland.

PMID: 15329867
DOI: 10.1002/rcm.1587

Abstract

An assay method with mass spectrometric detection was developed for the quantitative analysis of a pharmaceutical compound and its major metabolite in human plasma using chip-based infusion. Liquid-liquid extraction sample preparation was found to be essential to minimize matrix suppression and to achieve a limit of quantitation (LOQ) of 2.5 ng/mL using a 100 microL plasma aliquot. The potential for simultaneous quantitation in selected reaction monitoring (SRM), tandem mass spectrometry (MS/MS) (enhanced product ion), and MS(3) was investigated and found to be very beneficial in improving assay selectivity. A novel concept for monitoring quantitative assay performance using a SRM/MS(3) ratio is proposed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Mass Spectrometry / instrumentation*
Mass Spectrometry / methods*
Nanotechnology / methods*
Pharmaceutical Preparations / blood*
Reproducibility of Results
Sensitivity and Specificity

Substances

Pharmaceutical Preparations