Secretory IgA antibodies provide cross-protection against infection with different strains of influenza B virus

J Med Virol. 2004 Oct;74(2):328-35. doi: 10.1002/jmv.20173.


This study examined whether secretory IgA (S-IgA) antibodies (Abs) could confer cross-protective immunity against infection with influenza B viruses of antigenically distinct lineages. Wild-type or polymeric Ig receptor (pIgR)-knockout (KO) mice were immunized by infection with different B viruses or by intranasal (i.n.) administration with different inactivated vaccines. Four weeks later mice were challenged with either the B/Ibaraki/2/85 virus, representative of the B/Victoria/2/87 (B/Victoria)-lineage, or B/Yamagata/16/88 virus, representative of the B/Yamagata-lineage. Three days after challenge, nasal wash and serum specimens were assayed for IgA and IgG Abs specific for challenge viral antigens and for protection against challenge viruses. In wild-type mice, B/Ibaraki (or B/Yamagata) cross-reactive IgA Abs were detected at higher levels when infected or immunized with homologous-lineage viruses and at lower levels when infected or immunized with heterologous-lineage viruses. There was a correlation between the amount of nasal cross-reactive IgA Ab and the efficacy of cross-protection with a homologous-lineage virus. In mice lacking the pIgR, nasal cross-protective IgA Abs were only marginally detected in vaccinated mice and an accumulation of IgA in the serum was observed. This reduction of nasal IgA was accompanied by inefficient cross-protection against the B/Ibaraki (or B/Yamagata) virus infection. These results suggest that challenge viral-antigen cross-reactive S-IgA in nasal secretions induced by i.n. infection or vaccination is involved in providing cross-protection against challenge infection with virus within either the B/Victoria- or B/Yamagata-lineage.

MeSH terms

  • Animals
  • Female
  • Humans
  • Immunoglobulin A, Secretory / immunology*
  • Immunoglobulin G / blood
  • Influenza B virus / classification*
  • Influenza B virus / genetics
  • Influenza B virus / immunology*
  • Influenza Vaccines / immunology
  • Influenza, Human / immunology*
  • Influenza, Human / prevention & control*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Receptors, Polymeric Immunoglobulin / genetics
  • Vaccines, Inactivated / immunology


  • Immunoglobulin A, Secretory
  • Immunoglobulin G
  • Influenza Vaccines
  • Receptors, Polymeric Immunoglobulin
  • Vaccines, Inactivated