In vitro control of fasciation in proliferating nucellar embryos of Mangifera indica L. var totapari red small for cloning

Indian J Exp Biol. 2003 Nov;41(11):1311-6.

Abstract

Nucellar tissue contained in ovular halves of young fruits of Mangifera indica L. totapari red small, a dwarfing rootstock, differentiated fasciated embryonal structures in presence of 6-benzylaminopurine [BAP(0.15 mg l(-1))], 6-(gamma-gamma-dimethylallylamino) purine [2iP(0.15 mg l(-1))] and indole-3-acetic acid [(IAA(0.5 mg l(-1))] incorporated in the semisolid medium during 50-60 days. Due to embryonal fasciation, hardly 2-3 well-formed embryos could be obtained per culture of proliferating embryos. Of the 3 ethylene inhibitors [L-alpha-(2-aminoethoxyvinyl)-glycine-HCl (AVG), AgNO3 and salicylic acid (SA)] used, embryonal fasciation and necrosis of intervening tissue was completely controlled by 3-4 subcultures of fasciated mass of embryos under the influence of AVG (0.05 mg l(-1)) in presence of adenine sulphate [AdS (50 mg l(-1))] incorporated in the same medium. Almost synchronized development of isolated embryos, measuring ca 2 cm in length, was observed in a different medium used in liquid stationary state and supplemented, particularly with stress-producing substances [abscisic acid (ABA, 0.01 mg l(-1)); and polyethylene glycol (PEG, 100 mg l(-1))] besides certain other modifications. About 34% convertibility of processed embryos was obtained during a period of 90 days. The plantlets had well-developed roots along with laterals which were longer than leafy shoots. In vitro raised plants survived ex vitro for about 2 months.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abscisic Acid / pharmacology
  • Adenine / analogs & derivatives*
  • Adenine / pharmacology
  • Benzyl Compounds
  • Ethylenes / antagonists & inhibitors
  • Germination / drug effects
  • In Vitro Techniques
  • Indoleacetic Acids / pharmacology
  • Kinetin
  • Mangifera / drug effects*
  • Mangifera / embryology*
  • Mangifera / physiology
  • Plant Growth Regulators / pharmacology*
  • Polyethylene Glycols / pharmacology
  • Purines / pharmacology
  • Regeneration / drug effects*
  • Seeds / drug effects
  • Seeds / growth & development
  • Surface-Active Agents / pharmacology

Substances

  • Benzyl Compounds
  • Ethylenes
  • Indoleacetic Acids
  • Plant Growth Regulators
  • Purines
  • Surface-Active Agents
  • Polyethylene Glycols
  • indoleacetic acid
  • Abscisic Acid
  • ethylene
  • Adenine
  • benzylaminopurine
  • Kinetin