Palmitoylputrescine, an antibiotic isolated from the heterologous expression of DNA extracted from bromeliad tank water

J Nat Prod. 2004 Aug;67(8):1283-6. doi: 10.1021/np0499766.


Heterologous expression of large fragments of microbial DNA extracted directly from environmental samples (environmental DNA, or eDNA) in easily cultured hosts should provide access to some of the natural products produced by previously uncultured bacteria. The natural product antibiotic palmitoylputrescine (1) was isolated from Escherichia coli transformed with a cosmid (pCSLF16) containing DNA extracted directly from Costa Rican bromeliad tank water. In this report we describe the characterization of this antibiotic and its biosynthetic gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anti-Bacterial Agents / isolation & purification*
  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / pharmacology
  • Bacillus subtilis / drug effects
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / genetics*
  • Escherichia coli / genetics*
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Molecular Structure
  • Putrescine / analogs & derivatives
  • Putrescine / isolation & purification*
  • Putrescine / metabolism
  • Putrescine / pharmacology
  • Water


  • Anti-Bacterial Agents
  • DNA, Bacterial
  • palmitoylputrescine
  • Water
  • Putrescine