Inhibition of p53 transactivation required for transformation by adenovirus early 1B protein

Nature. 1992 May 7;357(6373):82-5. doi: 10.1038/357082a0.

Abstract

The cellular phosphoprotein p53 inhibits progression through the mammalian cell cycle. Both p53 alleles are frequently mutated in human tumours, indicating that p53 is a tumour suppressor. Recent studies have suggested that p53 functions as a transcriptional activator, but the significance of this activity in cell-cycle control has not been established. The adenovirus 2 (Ad2) early 1B (E1B) 55K protein binds to p53 in transformed cells and contributes to oncogenic transformation by Ad2 (refs 10-12). Here we report that mutants of E1B 55K and wild-type Ad12 E1B 54K proteins show a strong correlation between their ability to inhibit p53-mediated transcriptional activation and their ability to cooperate with adenovirus E1A protein in the transformation of primary cells. These results indicate that p53 probably inhibits cell cycling by functioning as a transcription factor.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenovirus Early Proteins
  • Animals
  • Cell Transformation, Viral / physiology*
  • Cells, Cultured
  • Gene Expression
  • Mutation
  • Oncogene Proteins, Viral / genetics
  • Oncogene Proteins, Viral / physiology*
  • Protein Binding
  • Rats
  • Structure-Activity Relationship
  • Transcriptional Activation
  • Tumor Suppressor Protein p53 / antagonists & inhibitors
  • Tumor Suppressor Protein p53 / physiology*

Substances

  • Adenovirus Early Proteins
  • Oncogene Proteins, Viral
  • Tumor Suppressor Protein p53