Diversity within Borrelia burgdorferi sensu lato genospecies in Switzerland by recA gene sequence

FEMS Microbiol Lett. 2004 Sep 1;238(1):115-23. doi: 10.1016/j.femsle.2004.07.025.

Abstract

A total of 874 Ixodes ricinus ticks were collected in Switzerland to investigate the genetic diversity of the Borrelia population. We integrated to the RT-PCR method the DNA sequence analysis of a 162-bp fragment of the recA gene. Five genospecies were detected: Borrelia afzelii, Borrelia burgdorferi s.s., Borrelia garinii, Borrelia valaisiana, and Borrelia lusitaniae. A heterogeneous distribution was observed within the B. burgdorferi s.l. genospecies. The most prevalent and diverse genospecies found in Switzerland was Borrelia afzelii, which might suggest a rapid evolution of this genospecies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Borrelia burgdorferi Group / classification*
  • Borrelia burgdorferi Group / genetics*
  • Borrelia burgdorferi Group / isolation & purification
  • Cats
  • Cattle
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / isolation & purification
  • Dogs
  • Genetic Variation
  • Goats
  • Humans
  • Ixodes / microbiology*
  • Larva / microbiology
  • Molecular Epidemiology
  • Molecular Sequence Data
  • Nymph / microbiology
  • Phylogeny
  • Rec A Recombinases / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology
  • Switzerland

Substances

  • DNA, Bacterial
  • Rec A Recombinases

Associated data

  • GENBANK/AY586362
  • GENBANK/AY586363
  • GENBANK/AY586364
  • GENBANK/AY586365
  • GENBANK/AY586366
  • GENBANK/AY586367
  • GENBANK/AY586368
  • GENBANK/AY586369
  • GENBANK/AY586370
  • GENBANK/AY586371
  • GENBANK/AY586372
  • GENBANK/AY586373
  • GENBANK/AY586374
  • GENBANK/AY586375
  • GENBANK/AY586376
  • GENBANK/AY586377
  • GENBANK/AY586378
  • GENBANK/AY586379
  • GENBANK/AY586380
  • GENBANK/AY586381
  • GENBANK/AY586382
  • GENBANK/AY586383
  • GENBANK/AY586384
  • GENBANK/AY586385
  • GENBANK/AY586386
  • GENBANK/AY586387
  • GENBANK/AY586388
  • GENBANK/AY586389
  • GENBANK/AY586390
  • GENBANK/AY586391
  • GENBANK/AY586392