The ABC's (and XYZ's) of peptide sequencing

Nat Rev Mol Cell Biol. 2004 Sep;5(9):699-711. doi: 10.1038/nrm1468.


Proteomics is an increasingly powerful and indispensable technology in molecular cell biology. It can be used to identify the components of small protein complexes and large organelles, to determine post-translational modifications and in sophisticated functional screens. The key - but little understood - technology in mass-spectrometry-based proteomics is peptide sequencing, which we describe and review here in an easily accessible format.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acid Sequence
  • Computational Biology
  • Databases, Factual
  • Mass Spectrometry / instrumentation
  • Mass Spectrometry / methods
  • Molecular Structure
  • Peptides* / analysis
  • Peptides* / genetics
  • Protein Isoforms / analysis
  • Protein Isoforms / genetics
  • Proteomics / methods*
  • Sequence Analysis, Protein / instrumentation
  • Sequence Analysis, Protein / methods*


  • Peptides
  • Protein Isoforms