Dynamics of yeast Myosin I: evidence for a possible role in scission of endocytic vesicles

Curr Biol. 2004 Sep 7;14(17):1604-9. doi: 10.1016/j.cub.2004.08.055.

Abstract

Cortical actin patches are dynamic structures required for endocytosis in yeast. Recent studies have shown that components of cortical patches localize to the plasma membrane in a precisely orchestrated manner, and their movements at and away from the plasma membrane may define the endocytic membrane invagination and vesicle scission events, respectively. Here, through live-cell imaging, we analyze the dynamics of the highly conserved class I unconventional myosin, Myo5, which also localizes to cortical patches and is known to be involved in endocytosis and actin nucleation. Myo5 exhibits a pattern of dynamic localization different from all cortical patch components analyzed to date. Myo5 associates with cortical patches only transiently and remains stationary during its brief cortical lifespan. The peak of Myo5 association with cortical patches immediately precedes the fast movement of Arp2/3 complex-associated structures away from the plasma membrane, thus correlating precisely with the proposed vesicle scission event. To further test the role of Myo5, we generated a temperature-sensitive mutant myo5 allele. In the myo5 mutant cells, Myo5 exhibits a significantly extended cortical lifespan as a result of a general impairment of Myo5 function, and Arp2 patches exhibit an extended slow-movement phase prior to the fast movement toward the cell interior. The myo5 mutant cells are defective in fluid-phase endocytosis and exhibit an increased number of invaginations on the membrane. Based on these results, we hypothesize that the myosin I motor protein facilitates the membrane fusion/vesicle scission event of endocytosis.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actin-Related Protein 2
  • Actins / metabolism*
  • Blotting, Western
  • Endocytosis / physiology*
  • Microscopy, Electron
  • Mutation / genetics
  • Myosin Type I / genetics
  • Myosin Type I / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Transport Vesicles / metabolism*
  • Yeasts / metabolism*
  • Yeasts / ultrastructure

Substances

  • ARP2 protein, S cerevisiae
  • Actin-Related Protein 2
  • Actins
  • MYO5 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Myosin Type I