Abstract
The Sam68-like mammalian protein SLM-1 is a member of the STAR protein family and is related to SAM68 and SLM-2. Here, we demonstrate that rSLM-1 interacts with itself, scaffold-attachment factor B, YT521-B, SAM68, rSLM-2, SRp30c, and hnRNP G. rSLM-1 regulates splice site selection in vivo via a purine-rich enhancer. In contrast to the widely expressed SAM68 and rSLM-2 proteins, rSLM-1 is found primarily in brain and, to a much smaller degree, in testis. In the brain, rSLM-1 and rSLM-2 are predominantly expressed in different neurons. In the hippocampal formation, rSLM-1 is present only in the dentate gyrus, whereas rSLM-2 is found in the pyramidal cells of the CA1, CA3, and CA4 regions. rSLM-1, but not rSLM-2, is phosphorylated by p59(fyn). p59(fyn)-mediated phosphorylation abolishes the ability of rSLM-1 to regulate splice site selection, but has no effect on rSLM-2 activity. This suggests that rSLM-1-positive cells could respond with a change of their splicing pattern to p59(fyn) activation, whereas rSLM-2-positive cells would not be affected. Together, our data indicate that rSLM-1 is a tissue-specific splicing factor whose activity is regulated by tyrosine phosphorylation signals emanating from p59(fyn).
Copyright 2004 Elsevier Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Animals
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Brain / metabolism*
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DNA, Complementary / analysis
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DNA, Complementary / genetics
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Enhancer Elements, Genetic / genetics
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Heterogeneous-Nuclear Ribonucleoproteins / genetics
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Heterogeneous-Nuclear Ribonucleoproteins / metabolism
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Hippocampus / metabolism
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Matrix Attachment Region Binding Proteins / metabolism
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Nuclear Matrix-Associated Proteins / metabolism
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Organ Specificity / genetics
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Phosphoproteins / genetics
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Phosphoproteins / metabolism
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Phosphorylation
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-fyn
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Pyramidal Cells / metabolism
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RNA Splice Sites / genetics
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RNA Splicing / genetics*
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RNA Splicing Factors
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / isolation & purification
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RNA-Binding Proteins / metabolism*
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Rats
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Receptors, Estrogen / metabolism
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Serine-Arginine Splicing Factors
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src-Family Kinases / genetics
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src-Family Kinases / isolation & purification
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src-Family Kinases / metabolism*
Substances
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Adaptor Proteins, Signal Transducing
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DNA, Complementary
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Heterogeneous-Nuclear Ribonucleoproteins
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KHDRBS3 protein, human
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Khdrbs1 protein, mouse
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Khdrbs2 protein, mouse
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Khdrbs2 protein, rat
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Khdrbs3 protein, rat
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Matrix Attachment Region Binding Proteins
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Nerve Tissue Proteins
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Nuclear Matrix-Associated Proteins
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Nuclear Proteins
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Phosphoproteins
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Proto-Oncogene Proteins
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RNA Splice Sites
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RNA Splicing Factors
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RNA-Binding Proteins
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Receptors, Estrogen
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SAFB protein, human
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Ythdc1 protein, rat
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Serine-Arginine Splicing Factors
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Fyn protein, rat
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Proto-Oncogene Proteins c-fyn
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src-Family Kinases
Associated data
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GENBANK/AF305618
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GENBANK/AF305619