The monoclonal antibody MEL-14 can block lymphocyte migration into a site of chronic inflammation

Eur J Immunol. 1992 Jun;22(6):1647-50. doi: 10.1002/eji.1830220646.

Abstract

The effect of MEL-14, a monoclonal antibody which binds to the lymphocyte homing receptor (MEL-14 Ag) on lymphocytes for peripheral lymph node (PLN) high endothelial venules (HEV), was investigated on lymphocyte migration into a delayed-type hypersensitivity (DTH)-like lesion produced by sensitization and challenge to Bordetella pertussis vaccine (BPV). Pretreatment of lymphocytes with saturating concentrations of MEL-14 caused a highly significant inhibition of lymphocyte migration into the chronically inflamed site and PLN. This finding suggests that lymphocyte migration into the BPV-induced site of chronic inflammation may be regulated by the same mechanism as lymphocyte migration into the PLN. It further indicates that the ligand for the MEL-14 Ag adhesion molecule, recognized by lymphocyte which home to the PLN, may also be expressed on HEV-like vessels at sites of BPV-induced chronic inflammation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibody Specificity
  • Antigens, CD / physiology
  • Blood Physiological Phenomena
  • Cell Migration Inhibition
  • Cell Movement / immunology
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Histocompatibility Antigens / physiology
  • Hypersensitivity, Delayed / immunology
  • Inflammation / immunology*
  • Leukocyte Common Antigens
  • Liver / physiology
  • Lymph Nodes / immunology
  • Lymph Nodes / physiology
  • Lymphocytes / physiology*
  • Mice
  • Mice, Inbred BALB C
  • Pertussis Vaccine / immunology
  • Peyer's Patches / physiology
  • Receptors, Lymphocyte Homing / physiology*
  • Spleen / physiology
  • Thymus Gland / physiology

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Histocompatibility Antigens
  • Pertussis Vaccine
  • Receptors, Lymphocyte Homing
  • Leukocyte Common Antigens