Induction and functional analysis of two reduced nicotinamide adenine dinucleotide phosphate-dependent glutathione peroxidase-like proteins in Synechocystis PCC 6803 during the progression of oxidative stress

Plant Physiol. 2004 Sep;136(1):2855-61. doi: 10.1104/pp.104.044842. Epub 2004 Sep 3.


Synechocystis PCC 6803 contains two types of glutathione peroxidase-like proteins (GPX-1 and GPX-2) that utilize NADPH but not reduced glutathione and unsaturated fatty acid hydroperoxides or alkyl hydroperoxides. The steady-state transcript level of gpx-1 gradually increased under oxidative stress conditions imposed by high light intensity, high salinity, or application of methylviologen or t-butyl hydroperoxide in the wild-type and GPX-2 knock-out mutant (gpx-2Delta) cells. To examine the ability of GPX-1, GPX-2, and thioredoxin peroxidase to scavenge lipid hydroperoxide in vivo, we measured the photosynthetic evolution of O(2) and the level of lipid peroxidation in the wild-type and each type of mutant cell after the application of t-butyl hydroperoxide or H(2)O(2). The data reported here indicate that GPX-1 and GPX-2 are essential for the removal of lipid hydroperoxides under normal and stress conditions, leading to the protection of membrane integrity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Base Sequence
  • DNA, Bacterial / genetics
  • Gene Targeting
  • Genes, Bacterial
  • Glutathione Peroxidase / biosynthesis*
  • Glutathione Peroxidase / genetics
  • Glutathione Peroxidase / metabolism
  • Glutathione Peroxidase GPX1
  • Isoenzymes / biosynthesis
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • Lipid Peroxidation
  • Molecular Sequence Data
  • Mutation
  • NADP / metabolism
  • Oxidative Stress
  • Oxygen / metabolism
  • Synechocystis / genetics
  • Synechocystis / metabolism*


  • Bacterial Proteins
  • DNA, Bacterial
  • Isoenzymes
  • NADP
  • Glutathione Peroxidase
  • Oxygen
  • Glutathione Peroxidase GPX1

Associated data

  • GENBANK/BAA17881
  • GENBANK/BAA18344