Abstract
It is important to understand which molecules are relevant for linking innate and adaptive immune cells. In this study, we show that OX40 ligand is selectively induced on IL-2, IL-12, or IL-15-activated human NK cells following stimulation through NKG2D, the low affinity receptor for IgG (CD16) or killer cell Ig-like receptor 2DS2. CD16-activated NK cells costimulate TCR-induced proliferation, and IFN-gamma produced by autologous CD4+ T cells and this process is dependent upon expression of OX40 ligand and B7 by the activated NK cells. These findings suggest a novel and unexpected link between the natural and specific immune responses, providing direct evidence for cross-talk between human CD4+ T cells and NK receptor-activated NK cells.
Copyright 2004 The American Association of Immunologists, Inc.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, CD / physiology
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Antigens, Surface
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B7-1 Antigen / metabolism
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B7-1 Antigen / physiology*
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B7-2 Antigen
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CD28 Antigens / metabolism
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CD4-Positive T-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / metabolism*
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Cell Communication / immunology*
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Cell Division / immunology
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Cell Line, Tumor
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Cytokines / blood
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Cytokines / physiology
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Humans
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Killer Cells, Natural / immunology
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Killer Cells, Natural / metabolism*
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Ligands
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Lymphocyte Activation / immunology*
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Membrane Glycoproteins / physiology
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Membrane Proteins
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Oligonucleotide Array Sequence Analysis
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Receptor Cross-Talk / immunology*
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Receptors, Antigen, T-Cell / physiology
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Receptors, Immunologic / blood
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Receptors, Immunologic / physiology
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Receptors, KIR
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Receptors, OX40
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Receptors, Tumor Necrosis Factor / metabolism
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Tumor Necrosis Factor-alpha / biosynthesis
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Tumor Necrosis Factor-alpha / metabolism*
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Tumor Necrosis Factor-alpha / physiology
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Up-Regulation / immunology
Substances
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Antigens, CD
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Antigens, Surface
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B7-1 Antigen
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B7-2 Antigen
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CD28 Antigens
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CD86 protein, human
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Cytokines
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Ligands
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Membrane Glycoproteins
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Membrane Proteins
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Receptors, Antigen, T-Cell
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Receptors, Immunologic
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Receptors, KIR
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Receptors, OX40
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Receptors, Tumor Necrosis Factor
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TNFRSF4 protein, human
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Tumor Necrosis Factor-alpha