Establishment of a novel anaplastic large-cell lymphoma-cell line (COST) from a 'small-cell variant' of ALCL

Leukemia. 2004 Oct;18(10):1693-8. doi: 10.1038/sj.leu.2403464.


Anaplastic large-cell lymphoma (ALCL) is a distinct biological and cytogenetic entity with a broad spectrum of morphological features (common type, small-cell variant and lymphohistiocytic variant). Few cell lines of ALCL are available and they all originate from primary tumors demonstrating the common type morphology (ie large-sized lymphoma cells). We established a new ALCL cell line (COST) from the peripheral blood of a patient with a small-cell variant of ALCL, at diagnosis. Cells growing in vitro and in SCID mice consisted of two populations, that is, small- and large-sized cells as seen in the patient's tumor. Both large and small malignant cells were positive for CD43/MT1 T-cell associated antigen, perforin, granzyme B and TIA-1, but negative for CD2, CD3, CD5, CD7, CD4 and CD8 antigens. Standard cytogenetic studies as well as multiplex FISH confirmed the presence of the canonical t(2;5)(p23;q35) translocation, but also revealed additional numerical and structural abnormalities. The COST cell line is the first ALCL small-cell variant cell line, and thus provides a potentially useful tool for further functional and molecular studies that should improve our understanding of the small-cell variant of ALCL, which is more frequently complicated by a leukemic phase.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD / metabolism
  • Child, Preschool
  • Chromosome Aberrations
  • Chromosomes, Human, Pair 2 / genetics
  • Chromosomes, Human, Pair 5 / genetics
  • Cytogenetic Analysis
  • Female
  • Gene Rearrangement, T-Lymphocyte
  • Humans
  • Immunophenotyping
  • In Situ Hybridization, Fluorescence
  • In Vitro Techniques
  • Lymphoma, Large-Cell, Anaplastic / genetics
  • Lymphoma, Large-Cell, Anaplastic / immunology
  • Lymphoma, Large-Cell, Anaplastic / pathology*
  • Male
  • Mice
  • Mice, SCID
  • Reverse Transcriptase Polymerase Chain Reaction
  • Translocation, Genetic
  • Tumor Cells, Cultured*


  • Antigens, CD