Application of real-time cell electronic sensing (RT-CES) technology to cell-based assays

Assay Drug Dev Technol. 2004 Aug;2(4):363-72. doi: 10.1089/adt.2004.2.363.


Label-free detection emerges as a new approach in the development of technologies for cell-based screening assays. Unlike the classic detection methods that use fluorescence, radioisotope, luminescence, or light absorption, label-free detection directly measures the cell function without using a labeled molecule. The advantages of label-free detection include a simple homogeneous assay format, noninvasive measurement, less interference with normal cell function, kinetic measurement, and reduced time for assay development. Here, we have applied the electrical impedance detection method in a real-time cell electronic sensing (RT-CES trade mark ) system for cell-based assays. The cell growth rate measured by this RT-CES system was comparable to actual cell number counted manually. In addition, cell proliferation, cytotoxicity, cytoprotection, cell growth inhibition, and apoptosis data generated by this RT-CES system correlated with those determined by the classic methods. The conclusion is that the RT-CES system is a useful tool for label-free detection of certain cell-based parameters.

Publication types

  • Comparative Study

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation
  • Computer Systems*
  • Dose-Response Relationship, Drug
  • Doxorubicin / pharmacology
  • Electrochemistry / instrumentation
  • Electrochemistry / methods*
  • Humans
  • Microelectrodes
  • Sensitivity and Specificity


  • Doxorubicin