Affinity purification of the tobacco plastid RNA polymerase and in vitro reconstitution of the holoenzyme

Plant J. 2004 Oct;40(1):164-72. doi: 10.1111/j.1365-313X.2004.02195.x.

Abstract

We affinity-purified the tobacco plastid-encoded plastid RNA polymerase (PEP) complex by the alpha subunit containing a C-terminal 12 x histidine tag using heparin and Ni(2+) chromatography. The composition of the complex was determined by mass spectrometry after separating the proteins of the >900 kDa complex in blue native and SDS polyacrylamide gels. The purified PEP contained the core alpha, beta, beta', beta" subunits and five major associated proteins of unknown function, but lacked sigma factors required for promoter recognition. The holoenzyme efficiently recognized a plastid psbA promoter when it was reconstituted from the purified PEP and recombinant plastid sigma factors. Reconstitution of a plastid holoenzyme with individual sigma factors will facilitate identification of sigma factor-specific promoter elements.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Chromatography, Affinity / methods
  • DNA-Directed RNA Polymerases / genetics
  • DNA-Directed RNA Polymerases / isolation & purification*
  • Molecular Sequence Data
  • Molecular Weight
  • Plant Proteins / genetics
  • Plant Proteins / isolation & purification
  • Plastids / enzymology*
  • Protein Subunits / isolation & purification
  • Tobacco / enzymology*
  • Tobacco / genetics
  • Tobacco / ultrastructure

Substances

  • Plant Proteins
  • Protein Subunits
  • DNA-Directed RNA Polymerases