Rolling blackout, a newly identified PIP2-DAG pathway lipase required for Drosophila phototransduction

Nat Neurosci. 2004 Oct;7(10):1070-8. doi: 10.1038/nn1313. Epub 2004 Sep 12.

Abstract

The rolling blackout (rbo) gene encodes an integral plasma membrane lipase required for Drosophila phototransduction. Photoreceptors are enriched for the RBO protein, and temperature-sensitive rbo mutants show reversible elimination of phototransduction within minutes, demonstrating an acute requirement for the protein. The block is activity dependent, indicating that the action of RBO is use dependent. Conditional rbo mutants show activity-dependent depletion of diacylglycerol and concomitant accumulation of phosphatidylinositol phosphate and phosphatidylinositol 4,5-bisphosphate within minutes of induction, suggesting rapid downregulation of phospholipase C (PLC) activity. The RBO requirement identifies an essential regulatory step in G-protein-coupled, PLC-dependent inositol lipid signaling mediating activation of TRP and TRPL channels during phototransduction.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence / genetics
  • Animals
  • Animals, Genetically Modified
  • Base Sequence / genetics
  • Carboxylic Ester Hydrolases / genetics
  • Carboxylic Ester Hydrolases / isolation & purification
  • Carboxylic Ester Hydrolases / metabolism*
  • Cell Membrane / enzymology
  • Chromosome Mapping
  • DNA, Complementary / analysis
  • DNA, Complementary / genetics
  • Diglycerides / metabolism*
  • Down-Regulation / genetics
  • Drosophila Proteins / genetics
  • Drosophila Proteins / isolation & purification
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / metabolism
  • Gene Expression Regulation / genetics
  • Membrane Potentials / genetics
  • Membrane Proteins / genetics
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism
  • Molecular Sequence Data
  • Mutation / genetics
  • Phosphatidylinositol 4,5-Diphosphate / metabolism*
  • Phospholipases / genetics
  • Phospholipases / isolation & purification
  • Phospholipases / metabolism*
  • Photoreceptor Cells, Invertebrate / enzymology
  • Temperature
  • Type C Phospholipases / metabolism
  • Vision, Ocular / genetics*

Substances

  • DNA, Complementary
  • Diglycerides
  • Drosophila Proteins
  • Membrane Proteins
  • Phosphatidylinositol 4,5-Diphosphate
  • Phospholipases
  • Carboxylic Ester Hydrolases
  • stmA protein, Drosophila
  • Type C Phospholipases
  • GTP-Binding Proteins