Protein kinase CK2 is coassembled with small conductance Ca(2+)-activated K+ channels and regulates channel gating

Neuron. 2004 Sep 16;43(6):847-58. doi: 10.1016/j.neuron.2004.08.033.

Abstract

Small conductance Ca(2+)-activated K+ channels (SK channels) couple the membrane potential to fluctuations in intracellular Ca2+ concentration in many types of cells. SK channels are gated by Ca2+ ions via calmodulin that is constitutively bound to the intracellular C terminus of the channels and serves as the Ca2+ sensor. Here we show that, in addition, the cytoplasmic N and C termini of the channel protein form a polyprotein complex with the catalytic and regulatory subunits of protein kinase CK2 and protein phosphatase 2A. Within this complex, CK2 phosphorylates calmodulin at threonine 80, reducing by 5-fold the apparent Ca2+ sensitivity and accelerating channel deactivation. The results show that native SK channels are polyprotein complexes and demonstrate that the balance between kinase and phosphatase activities within the protein complex shapes the hyperpolarizing response mediated by SK channels.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphate / pharmacology
  • Animals
  • Animals, Newborn
  • Blotting, Western / methods
  • Brain / cytology
  • Brain / metabolism
  • Calcium / metabolism*
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism
  • Calmodulin / metabolism
  • Casein Kinase II
  • Cell Membrane / metabolism
  • Chromatography, Affinity / methods
  • Cochlea / metabolism
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Electrophoresis, Gel, Two-Dimensional / methods
  • Immunohistochemistry / methods
  • Ion Channel Gating / physiology*
  • Magnesium / pharmacology
  • Mass Spectrometry / methods
  • Membrane Potentials / drug effects
  • Membrane Potentials / physiology
  • Mice
  • Mice, Transgenic
  • Microscopy, Confocal / methods
  • Mutagenesis / physiology
  • Mutation
  • Oocysts
  • Patch-Clamp Techniques / methods
  • Phosphoprotein Phosphatases / metabolism
  • Phosphorylation
  • Potassium Channels / metabolism*
  • Potassium Channels, Calcium-Activated*
  • Protein Binding
  • Protein Phosphatase 2
  • Protein Subunits / metabolism
  • Protein-Serine-Threonine Kinases / metabolism*
  • Rats
  • Small-Conductance Calcium-Activated Potassium Channels
  • Spermine / pharmacology
  • Synaptophysin / metabolism
  • Time Factors
  • Two-Hybrid System Techniques
  • Xenopus

Substances

  • Calmodulin
  • Potassium Channels
  • Potassium Channels, Calcium-Activated
  • Protein Subunits
  • Small-Conductance Calcium-Activated Potassium Channels
  • Synaptophysin
  • Spermine
  • Adenosine Triphosphate
  • Casein Kinase II
  • Protein-Serine-Threonine Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 2
  • Magnesium
  • Calcium